How assembly worksΒΆ

See hand outs.

This is shotgun sequencing of a common Mexican phrase. Can you assemble the original phrase?


  • no googling!
  • work in groups of 4-6


  • think about strategy; how would you do this for 10x, or 100x, the data?
  • does this get easier, or harder, with more people in a group?
  • what are strategies for verifying the results?
  • is it helpful that the “reads” are sorted?
  • what is the benefit of “paired end” reads?
  • what is the benefit of longer reads?
  • how problematic are errors in the reads?